nasC

nitrate reductase (catalytic subunit)

Locus: BSU_03310

Isoelectric point: 6.73

Molecular weight: 78.39 kDa

Protein length: 710 aa

Gene length: 2133 bp

Function: utilization of nitrate

Product: nitrate reductase (catalytic subunit)

Essential: no

Synonyms: narB,nasBB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of nitrate/ nitrite

Gene

Coordinates: 358,303 → 360,435

The protein

Protein family

• prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)

Domains

• 4Fe-4S Mo/W bis-MGD-type domain (aa 19-77) (according to UniProt)

Cofactors

• MoCo PubMed
• Fe-S cluster PubMed

Structure

• 2IV2 (from E. coli, 32% identity) PubMed

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE03310 (ΔnasC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA, downstream forward: _UP4_TAAATTTTTCATAAAATTTT
• BKK03310 (ΔnasC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA, downstream forward: _UP4_TAAATTTTTCATAAAATTTT

References

Reviews

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Original publications

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