nrgB [Wed Sep 23 2015 12:58:31 GMT+0200 (CEST)]

nitrogen-regulated PII protein

locus: BSU36520

pI: 8.00

mw: 12.00 kDa

function: regulation of ammonium uptake

product: nitrogen-regulated PII protein

essential: no

synonyms: glnK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|transcription factors and their control], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|TnrA regulon]

The protein

Protein family

• P(II) protein family (according to Swiss-Prot)

Structure

• [PDB|4R25]
• [http://pdb.org/pdb/search/structidSearch.do?structureId=4r25 4R25] [Pubmed|25691471]
• [http://www.ebi.ac.uk/pdbe-srv/view/entry/2nuu/summary 2NUU] (the complex with [protein|search|NrgA], from ''E. coli'') [Pubmed|17220269]

[SW|Localization]

• cell membrane, via [protein|search|NrgA] [Pubmed|14600241]

[SW|Interactions]

• [protein|search|NrgA]-[protein|search|NrgB] [Pubmed|14600241]
• [protein|search|TnrA]-[protein|search|NrgB] [Pubmed|17001076]
• [protein|search|GlnA]-[protein|search|NrgB] [Pubmed|21435182]

Expression and Regulation

Operon

• ''[protein|search|nrgA]-[protein|search|nrgB]'' [Pubmed|8282685]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8282685]

Regulation

• induced by ammonium limitation
• induced by ammonium limitation ([protein|search|TnrA]) [Pubmed|8799114]

Regulatory mechanism

• [protein|search|TnrA]: transcription activation [Pubmed|8799114]

Biological materials

Mutant

• GP259 (cat), GP255 (''[protein|search|nrgA]-[protein|search|nrgB]'', cat), both available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]

Antibody

• available in [SW|Jörg Stülke] lab

Labs working on this gene/protein

• [SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

References

Reviews

• 10637624,18182294,11238986,23847201

Original publications

• 23010999,12823818,8799114,17001076,14600241,8282685,1670935,17220269,23535029,23818625,21435182,25691471,25755103