nrgB

nitrogen-regulated PII protein

locus: BSU_36520

pI: 8.82

mw: 12.68 kDa

protein length: 116 aa

gene length: 351 bp

function: regulation of ammonium uptake

product: nitrogen-regulated PII protein

essential: no

synonyms: glnK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,758,016 → 3,758,366

The protein

Protein family

• P(II) protein family (single member, according to UniProt)

Structure

• [PDB|4R25] [Pubmed|25691471]
• [http://pdb.org/pdb/search/structidSearch.do?structureId=4r25 4R25] [Pubmed|25691471]
• [http://www.ebi.ac.uk/pdbe-srv/view/entry/2nuu/summary 2NUU] (the complex with [protein|796BD46066B1E6147048664FA78679B42FA9D628|NrgA], from ''E. coli'') [Pubmed|17220269]

[SW|Localization]

• cell membrane, via [protein|796BD46066B1E6147048664FA78679B42FA9D628|NrgA] [Pubmed|14600241]

Expression and Regulation

Operons

• genes: [gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]
  description: [Pubmed|8282685]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8282685], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|8799114], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • induced by ammonium limitation

Biological materials

Mutant

• GP259 (ΔnrgB::cat), available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]
• GP255 (''Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]''::cat), available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]
• BKE36520 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG
• BKK36520 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG

Expression vector

• pGP182, Expression of His₆-[protein|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|NrgB] from E. coli, available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]

Antibody

• available in [SW|Jörg Stülke]s lab [Pubmed|14600241]

labs

• [SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

References

Reviews

• 10637624,18182294,11238986,23847201

Original publications

• 23010999,12823818,8799114,17001076,14600241,8282685,1670935,17220269,23535029,23818625,21435182,25691471,25755103