nrgB

nitrogen-regulated PII protein

Locus: BSU_36520

Isoelectric point: 8.82

Molecular weight: 12.68 kDa

Protein length: 116 aa

Gene length: 351 bp

Function: regulation of ammonium uptake

Product: nitrogen-regulated PII protein

Essential: no

Synonyms: glnK

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,758,016 → 3,758,366

The protein

Protein family

• P(II) protein family (single member, according to UniProt)

Structure

• 4R25 PubMed
• 4R25 PubMed
• 2NUU (the complex with NrgA, from E. coli) PubMed

Localization

• cell membrane, via NrgA PubMed

Expression and Regulation

Operons

• Genes: nrgA-nrgB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • induced by ammonium limitation

Biological materials

Mutant

• GP259 (ΔnrgB::cat), available in Jörg Stülke's lab PubMed
• GP255 (ΔnrgA-nrgB::cat), available in Jörg Stülke's lab PubMed
• BKE36520 (ΔnrgB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG
• BKK36520 (ΔnrgB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG

Expression vector

• pGP182, Expression of His₆-NrgB from E. coli, available in Jörg Stülke's lab PubMed

Antibody

• available in Jörg Stülkes lab PubMed

Labs working on this gene/protein

• Susan Fisher, Boston, USA homepage

References

Reviews

Loading

Original publications

Loading