nsrR

nitric oxide-responsive regulator

Locus: BSU_09380

Isoelectric point: 7.75

Molecular weight: 16.47 kDa

Protein length: 146 aa

Gene length: 441 bp

Function: repression of ResD-ResE-dependent genes in the absence of nitric oxide (NO)

Product: transcriptional repressor

Essential: no

Synonyms: yhdE

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 1,013,411 → 1,013,851

The protein

Domains

• HTH rrf2-type domain (aa 2-133) (according to UniProt)

Cofactors

• Fe-S cluster, required for DNA-binding PubMed

Effectors of protein activity

• nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets PubMed
• [4Fe-4S]-NsrR binds around the -35 element of the nasD promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO PubMed

Structure

• 3LWF (from Listeria innocua, 29% identity)

Expression and Regulation

Operons

• Genes: nsrR
  Description: PubMed

Biological materials

Mutant

• MGNA-A692 (yhdE::erm), available at the NBRP B. subtilis, Japan
• BKE09380 (ΔnsrR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA, downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
• BKK09380 (ΔnsrR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA, downstream forward: _UP4_TAGATTAAGATTCCTTCTTT

References

Reviews

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The NsrR regulon

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Other original publications

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