fpbO

petrobactin (3.4-catecholate siderophore) ABC transporter (permease)

Locus: BSU_03810

Isoelectric point: 9.73

Molecular weight: 35.32 kDa

Protein length: 315 aa

Gene length: 948 bp

Function: acquisition of iron

Product: petrobactin ABC transporter (permease)

Essential: no

Synonyms: yclO

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
• Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
• Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
• Groups of genes → Membrane proteins

Gene

Coordinates: 433,315 → 434,262

The protein

Catalyzed reaction/ biological activity

• uptake of the siderophore petrobactin PubMed

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• FecCD subfamily (according to UniProt)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: fpbN-fpbO-fpbP-fpbQ
  Description: PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed

Biological materials

Mutant

• MGNA-C066 (yclO::erm), available at the NBRP B. subtilis, Japan
• BKE03810 (ΔfpbO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA
• BKK03810 (ΔfpbO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA

References

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