fpbO

petrobactin (3.4-catecholate siderophore) [SW|ABC transporter] (permease)

locus: BSU_03810

pI: 9.73

mw: 35.32 kDa

protein length: 315 aa

gene length: 948 bp

function: acquisition of iron

product: petrobactin [SW|ABC transporter] (permease)

essential: no

synonyms: yclO

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 433,315 → 434,262

The protein

Catalyzed reaction/ biological activity

• uptake of the siderophore petrobactin [Pubmed|19955416]

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
• [SW|FecCD subfamily] (according to UniProt)

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
  description: [Pubmed|19955416]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]

Biological materials

Mutant

• MGNA-C066 (yclO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2064 NBRP B. subtilis, Japan]
• BKE03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA
• BKK03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA

References

• 10092453,19955416,12354229,29133393