rpoB

[SW|RNA polymerase] beta subunit

locus: BSU_01070

pI: 4.73

mw: 133.38 kDa

protein length: 1193 aa

gene length: 3582 bp

function: [SW|transcription]

product: [SW|RNA polymerase] beta subunit

essential: yes

ec: 2.7.7.6

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 121,919 → 125,500

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate + RNA_(n) --> diphosphate + RNA_{(n + 1)} (according to UniProt)

Protein family

• [SW|RNA polymerase] beta chain family (single member, according to UniProt)

Modification

• phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 [Pubmed|22517742]

Structure

• [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 59% identity) [Pubmed|19903881]
• [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|RpoB] beta1 domain) [Pubmed|20702425]

[SW|Localization]

• cytoplasm

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7657605], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

lacZ fusion

• pGP2683 (cat) based on [SW|pAC5], available in [SW|Jörg Stülke]'s lab
• pGP2682 (aphA3) based on [SW|pAC7], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

FLAG-tag construct

• GP2043 ''rpoB-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 22210308,24763425,28657884

Original publications

• 19680289,17644585,24491578,7657605,12486038,10675340,7592585,20525796,17981983,18763711,19191541,21512256,21821766,22517742,24572018,22484477,20702425,20817769,26293966,26538577,19903881,26098117,28539362,29794222,33068413