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pbpB [2018-02-16 17:07:21]
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pbpB [2018-02-16 17:07:21]

class B penicillin-binding protein 2B, Pbp2B, required for the assembly of the cell division complex and septal cell wall synthesis
locus
BSU15160
pI
9.23
mw
79.12 kDa
protein length
716 aa Sequence Blast
gene length
2148 bp Sequence Blast
function
septation, cell division
product
penicillin-binding protein 2B
essential
yes
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,581,947 → 1,584,097

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • septal cell wall synthesis (this function can be taken over by [protein|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|PBP3]) [pubmed|28792086]
  • assembly of the cell division complex (this is the reason for PbpB essentiality) [pubmed|28792086]

    • Protein family

  • transpeptidase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|38FD9805815BBDDD83789F0F402C0FB221B65FF4|SpoVD]

    • [SW|Domains]

  • contains two C-terminal [SW|PASTA domain]s (aa 598-657, 658-714)

    • Structure

  • [PDB|1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) [Pubmed|14734544]

    • [SW|Localization]

  • cell membrane [Pubmed|23869552,20870765]
  • localizes to the division site, this depends on [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW] [Pubmed|27895631,23895585]
  • extracellular (signal peptide) [Pubmed|18957862]
  • localizes to the sporulation septum during sporulation [Pubmed|15758244]

    • Expression and Regulation

    Operons

    genes
    [gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|mraW]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
    description
    [Pubmed|8636036,8244929]

    regulation

  • constitutively expressed [Pubmed|15758244]
    • view in new tab

    Biological materials

    GFP fusion

  • 3122 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]::pSG5061 (cat Pxyl-gfpa-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]1-825) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • FLAG-tag construct

  • GP2003 ''pbpB-3xFLAG spec'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

  • 8636036,14731276,8244929,20487272,10652091,19429628,18957862,21219466,20870765,14734544,23895585,24655324,27895631,28792086