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pbpB
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pbpB

class B penicillin-binding protein 2B, Pbp2B, required for the assembly of the cell division complex and septal cell wall synthesis
Locus
BSU_15160
Isoelectric point
9.23
Molecular weight
79.12 kDa
Protein length
716 aa Sequence Blast
Gene length
2151 bp Sequence Blast
Function
septation, cell division
Product
penicillin-binding protein 2B, member of the divisome
Essential
yes
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,581,947 → 1,584,097

Phenotypes of a mutant

  • essential PubMed
  • deletion of the PASTA domains is possible, results in impaired cell division and heat sensitivity PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • septal cell wall synthesis (this function can be taken over by PBP3) PubMed
  • assembly of the cell division complex (this is the reason for PbpB essentiality) PubMed
  • Protein family

  • transpeptidase family (according to UniProt)
  • Paralogous protein(s)

    Domains

  • contains two C-terminal PASTA domains (aa 598-657, aa 658-714) (according to UniProt)
  • Structure

  • 1RP5 (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed
  • Localization

  • cell membrane PubMed
  • localizes to the division site, this depends on FtsW PubMed
  • extracellular (signal peptide) PubMed
  • localizes to the sporulation septum during sporulation PubMed
  • during vegetative growth: septal PubMed
  • Expression and Regulation

    Operons

    Description

    Regulation

  • constitutively expressed PubMed
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    Biological materials

    GFP fusion

  • 3122 trpC2 pbpB::pSG5061 (cat Pxyl-gfpa-pbpB1-825) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC (due to essentiality of pbpB, the strain is xylose-dependent)
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • FLAG-tag construct

  • GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab
  • Labs working on this gene/protein

  • Jeff Errington, Newcastle University, UK homepage
  • References

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