pbpB

class B penicillin-binding protein 2B, Pbp2B, required for the assembly of the cell division complex and septal cell wall synthesis

Locus: BSU_15160

Isoelectric point: 9.23

Molecular weight: 79.12 kDa

Protein length: 716 aa

Gene length: 2151 bp

Function: septation, cell division

Product: penicillin-binding protein 2B, member of the divisome

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
• Cellular processes → Cell envelope and cell division → Cell division → Other genes
• Groups of genes → Essential genes
• Groups of genes → Secreted proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,581,947 → 1,584,097

Phenotypes of a mutant

• essential PubMed
• deletion of the PASTA domains is possible, results in impaired cell division and heat sensitivity PubMed

The protein

Catalyzed reaction/ biological activity

• septal cell wall synthesis (this function can be taken over by PBP3) PubMed
• assembly of the cell division complex (this is the reason for PbpB essentiality) PubMed

Protein family

• transpeptidase family (according to UniProt)

Paralogous protein(s)

• SpoVD

Domains

• contains two C-terminal PASTA domains (aa 598-657, aa 658-714) (according to UniProt)

Structure

• 1RP5 (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed

Localization

• cell membrane PubMed
• localizes to the division site, this depends on FtsW PubMed
• extracellular (signal peptide) PubMed
• localizes to the sporulation septum during sporulation PubMed
• during vegetative growth: septal PubMed

Expression and Regulation

Operons

• Genes: mraZ-mraW-ftsL-pbpB
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

GFP fusion

• 3122 trpC2 pbpB::pSG5061 (cat P_xyl-gfpa-pbpB1-825) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC (due to essentiality of pbpB, the strain is xylose-dependent)

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

FLAG-tag construct

• GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab

Labs working on this gene/protein

• Jeff Errington, Newcastle University, UK homepage

References

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