btr [2017-8-11 16:0:3]

transcriptional activator (AraC family), (AraC family DNA-binding domain fused to FeuA-like substrate-binding domain), regulation of the feuA-feuB-feuC-ybbA operon

locus: BSU01640

pI: 6.43

mw: 60.59 kDa

protein length: 529 aa

gene length: 1587 bp

function: regulation of iron acquisition

product: transcription activator (AraC family)

essential: no

synonyms: ybbB, yzbC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 183,414 → 185,003

The protein

Catalyzed reaction/ biological activity

• binding of a direct repeat in the ''[gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]'' promoter region and transcription activation of the ''[gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA]'' operon in the presence of bacillibactin or enterobactin [Pubmed|17725565,22210890]

Protein family

• [SW|AraC family]

[SW|Cofactors]

• enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

Effectors of protein activity

• enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

Expression and Regulation

Operons

• genes: [gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]
  description: [Pubmed|12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • expressed under conditions of iron starvation ([protein|search|Fur]) [Pubmed|12354229]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-B949 (ybbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1948 NBRP B. subtilis, Japan]

References

• 12884008,17725565,22210890,12354229,23199363,23504016