yvcJ

GTPase, nucleotide-binding protein, similar to RNase adaptor protein

locus: BSU_34770

pI: 6.70

mw: 33.70 kDa

protein length: 295 aa

gene length: 888 bp

function: required for the expression of late competence genes

product: GTPase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

Gene

Coordinates: 3,571,501 → 3,572,388

Phenotypes of a mutant

• reduced [SW|genetic competence] [Pubmed|19074378]

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], [gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]'') [Pubmed|19074378]

Protein family

• RapZ-like family (single member, according to UniProt)

Structure

• [PDB|5O5Q] (RapZ from E. coli, 41% identity) [pubmed|28977623]

[SW|Localization]

• can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium [Pubmed|21709426]

Additional information

• [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947727&log$=genesensor5&logdbfrom=pubmed Information on the ''E. coli'' homolog]

Expression and Regulation

Operons

• genes: [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
  description: [Pubmed|9237995]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16272399], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|search|trxB]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-B641 (yvcJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1640 NBRP B. subtilis, Japan]
• 1A885 ( ''yvcJ''::''cat''), [Pubmed|19074378], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC]
• BKE34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA
• BKK34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA

Expression vectors

• pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab

labs

• [SW|Anne Galinier], University of Marseille, France

References

Original publications

• 9237995,16272399,19074378,21709426,32994436

Publications on the ''E. coli'' homolog

• 17824929,28977623,24667238,23475961