SubtiBank SubtiBank
rpmH
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rpmH

ribosomal protein
locus
BSU_41060
pI
13.00
mw
5.00 kDa
protein length
44 aa Sequence Blast
gene length
135 bp Sequence Blast
function
translation
product
ribosomal protein L34
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

    • Gene

    Coordinates
    4,215,255 → 4,215,389

    Phenotypes of a mutant

  • severe slow-growth phenotype, suppressed by disruption of ''[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]'' or overexpression of ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]'' [Pubmed|25182490]
  • increased fraction of hyperpolarized cells (35.2% vs. 3.7% in the wild type) [pubmed|30853217]
  • the [SW|ribosome]s are destabilized [pubmed|25182490]

    • The protein

    Catalyzed reaction/ biological activity

  • required for efficient 70S-[SW|ribosome] formation [Pubmed|25182490]

    • Protein family

  • bacterial [SW|ribosomal protein] bL34 family (single member, according to UniProt)

    • Structure

  • [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

    • [SW|Localization]

  • [SW|ribosome]

    • Expression and Regulation

    Operons

    genes
    [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]
    description
    [Pubmed|2987847]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    genes
    [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]-[gene|594F2D36BE71A45303A33AA2A2931F56F0A969ED|rnpA]-[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • BKK41060 (Δ[gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK41060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGACACCTCCCTCG, downstream forward: _UP4_TAGGCCACTGAATAATGTCA

    • References

  • 19653700,2987847,2987848,20525796,23002217,25182490,25903689,30853217