yhdF

similar to glucose 1-dehydrogenase

Locus: BSU_09450

Isoelectric point: 5.16

Molecular weight: 31.36 kDa

Protein length: 289 aa

Gene length: 870 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 1,022,231 → 1,023,100

The protein

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Paralogous protein(s)

• YxbG, YvrD, BacC, YmfI, YdaD, FabG, YoxD, Gdh, FadH
• KduD:
• YkvO:
• YhxC:
• YhxD:
• YcdF:
• YjdA:
• FabL:
• YxbG (36.1%), BacC (37.8%), FabG (31.6%), YkvO (34.3%), KduD (33.1%), FadH (31.1%), YmfI (30,6%), YjdA (33.6%), YoxD (31.9%), FabL (30.1%), YvrD (30.2%)

Structure

• 3I30 (from Bacillus anthracis str. amesAncestor, 66% identity)

Expression and Regulation

Operons

• Genes: yhdF
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • induced by stress (SigB) PubMed

• Genes: citA-yhdF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CitR: repression, PubMed, in CitR regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-B478 (yhdF::erm), available at the NBRP B. subtilis, Japan
• BKE09450 (ΔyhdF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATTCATCACTCCTAGAC, downstream forward: _UP4_TAATCAACGAAAAACCAGCT
• BKK09450 (ΔyhdF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATTCATCACTCCTAGAC, downstream forward: _UP4_TAATCAACGAAAAACCAGCT

References

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