SubtiBank SubtiBank
ycbK [2019-06-18 09:38:59]
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ycbK [2019-06-18 09:38:59]

similar to efflux system
locus
BSU02540
pI
9.57
mw
33.81 kDa
protein length
312 aa Sequence Blast
gene length
939 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    277,342 → 278,280

    The protein

    Protein family

  • DUF6 domain (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|354C71C2F6AFE620866D90595E6AC6CEFA5705C3|rtpA]-[gene|EAE919A6088F1A455B6FCE62BA8CCBD05C1F98D5|ycbK]
    description
    [Pubmed|10706627]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10706627], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|T-box|T-box]: transcription termination/ antitermination, [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB] [pubmed|10706627], in [regulon|T-box|T-box]

    • regulation

  • ''[protein|search|rtpA]'': induced by tryptophan limitation ([protein|search|T-box] in the mRNA leader) [Pubmed|10706627]
    • view in new tab

    Other regulations

  • [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]: translation inhibition,

    • Biological materials

    Mutant

  • MGNA-C033 (ycbK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2031 NBRP B. subtilis, Japan]
  • BKE02540 (Δ[gene|EAE919A6088F1A455B6FCE62BA8CCBD05C1F98D5|ycbK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT, downstream forward: _UP4_TAATTTTTATAAAACACACA
  • BKK02540 (Δ[gene|EAE919A6088F1A455B6FCE62BA8CCBD05C1F98D5|ycbK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT, downstream forward: _UP4_TAATTTTTATAAAACACACA

    • References

  • 19258532,10706627,16879415