speB [2019-08-12 11:54:11]

agmatinase

locus: BSU_37490

pI: 4.77

mw: 32.26 kDa

protein length: 290 aa

gene length: 873 bp

function: spermidine, polyamine biosynthesis

product: agmatinase

essential: no

ec: 3.5.3.11

synonyms: ywhG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.6|Metabolism of polyamines]

Gene

Coordinates: 3,847,853 → 3,848,725

The protein

Catalyzed reaction/ biological activity

• Agmatine + H₂O = putrescine + urea (according to Swiss-Prot)

Protein family

• arginase family (with [protein|CD121BBF1DC206D1189ECF8B32456641A55C41D4|RocF] and [protein|FBB15E07B7134C57208EA82D5339708385DBC5FB|HutG], according to UniProt)

Structure

• [PDB|3LHL] (from Clostridium difficile, 41% identity)

Expression and Regulation

Operons

• genes: [gene|4F3E96C741561746B4E0EEA5AE29DB124C2F7249|speE]-[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]
  description: [Pubmed|9723923]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9723923], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-A522 (ywhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/522 NBRP B. subtilis, Japan]
• BKE37490 (Δ[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT
• BKK37490 (Δ[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT

References

• 9723923