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speB [2019-08-12 11:54:11]
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speB [2019-08-12 11:54:11]

agmatinase
Locus
BSU_37490
Isoelectric point
4.77
Molecular weight
32.26 kDa
Protein length
290 aa Sequence Blast
Gene length
873 bp Sequence Blast
Function
spermidine, polyamine biosynthesis
Product
agmatinase
Essential
no
E.C.
3.5.3.11
Synonyms
ywhG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Metabolism of polyamines

    • Gene

    Coordinates
    3,847,853 → 3,848,725

    The protein

    Catalyzed reaction/ biological activity

  • Agmatine + H2O = putrescine + urea (according to Swiss-Prot)

    • Protein family

  • arginase family (with RocF and HutG, according to UniProt)

    • Structure

  • 3LHL (from Clostridium difficile, 41% identity)

    • Expression and Regulation

    Operons

    Genes
    speE-speB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • MGNA-A522 (ywhG::erm), available at the NBRP B. subtilis, Japan
  • BKE37490 (ΔspeB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT
  • BKK37490 (ΔspeB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT

    • References

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