spoVG

RNA-binding regulatory protein, negative effector of asymetric septation at the onset of sporulation

locus: BSU_00490

pI: 5.11

mw: 10.75 kDa

protein length: 97 aa

gene length: 294 bp

function: cell division, control of sporulation initiation

product: negative effector of asymetric septation

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 55,866 → 56,159

The protein

Protein family

• SpoVG family (single member, according to UniProt)

Modification

• phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) [Pubmed|17218307]

Structure

• [PDB|2IA9]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]
  description: [Pubmed|408013]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|408013], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|2437099], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|16430695]

Biological materials

Mutant

• MGNA-A082 (spoVG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/82 NBRP B. subtilis, Japan]
• GP2109 (''[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]''::''tet'', available in [SW|Jörg Stülke]'s lab)
• BKE00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
• BKK00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA

Expression vector

• pGP2331: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab

Expression vectors

• pGP2163: expression of SpoVG-Strep by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• pGP2310: expression of SpoVG-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

References

• 408013,1850083,16430695,6405278,6411929,10348850,7559352,8419299,3100810,2437099,17218307,20803133,27048798