ycsA

putative tartrate dehydrogenase

locus: BSU_04000

pI: 5.11

mw: 38.67 kDa

protein length: 354 aa

gene length: 1065 bp

function: unknown

product: putative tartrate dehydrogenase

essential: no

ec: 1.1.1.93

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 452,830 → 453,894

The protein

Catalyzed reaction/ biological activity

• Tartrate + NAD⁺ --> oxaloglycolate + NADH (according to UniProt)

Protein family

• Isocitrate and isopropylmalate dehydrogenases family (with [protein|CB7D0E32C044D541CD966DC1F9DA489D518A7703|Icd] and [protein|105E3452D7F142A7D7616E35AF3FB753C9B63E38|LeuB], according to UniProt)

Paralogous protein(s)

• [protein|105E3452D7F142A7D7616E35AF3FB753C9B63E38|LeuB], [protein|CB7D0E32C044D541CD966DC1F9DA489D518A7703|Icd]

Structure

• [PDB|3FMX] (from ''Pseudomonas Putida '', 51% identity)

Expression and Regulation

Operons

• genes: [gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C020 (ycsA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2018 NBRP B. subtilis, Japan]
• BKE04000 (Δ[gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT, downstream forward: _UP4_TGATGAATCAGGCCGGTGGC
• BKK04000 (Δ[gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT, downstream forward: _UP4_TGATGAATCAGGCCGGTGGC