glpQ

glycerolphosphate diester phosphodiesterase, degrades wall teichoic acid during phosphate starvation

locus: BSU_02130

pI: 9.26

mw: 32.81 kDa

protein length: 293 aa

gene length: 882 bp

function: glycerol-3-phosphate utilization, degradation of wall teichoic acid during phosphate starvation

product: glycerolphosphate diester phosphodiesterase

essential: no

ec: 3.1.4.46

synonyms: ybeD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.3|Utilization of glycerol/ glycerol-3-phosphate]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.1|Utilization of phospholipids]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 233,014 → 233,895

The protein

Catalyzed reaction/ biological activity

• exohydrolytic activity on wall teichoic acid, acts in concert with [protein|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|PhoD] [Pubmed|27780866]
• removes glycerolphosphates from the free end of the unsubstituted wall teichoic acid up to the peptidoglycan linker [pubmed|32047114]
• sn-glycero-3-phosphoester + H₂O --> alcohol + H⁺ + sn-glycerol 3-phosphate (according to UniProt)

Protein family

• glycerophosphoryl diester phosphodiesterase family (with [protein|04CACAF4F786090AB67D7C606C944F07B7A7FE55|YhdW], according to UniProt)

Paralogous protein(s)

• [protein|04CACAF4F786090AB67D7C606C944F07B7A7FE55|YhdW], [protein|AF37A0E08AAAA9159DD1F0183A61713EF582E830|YqiK]

[SW|Domains]

• GP-PDE domain (aa 38-290) (according to UniProt)

Structure

• [PDB|5T9B] [Pubmed|27780866]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]-[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]
  description: [Pubmed|8012593]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8012593], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP]: antitermination, via a protein-dependent [SW|RNA switch] [Pubmed|8012593], in [regulon|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|10913081], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by glycerol ([protein|search|GlpP]) [Pubmed|8012593]

• genes: [gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]

  regulation

  • induced by glycerol ([protein|search|GlpP]) [Pubmed|8012593]

Biological materials

Mutant

• BKE02130 (Δ[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG
• BKK02130 (Δ[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG

References

Reviews

• 24373430

Original publications

• 8012593,10913081,18214974,18957862,27780866,32047114