sufC

ABC transporter (ATP-binding protein), synthesis of Fe-S clusters

Locus: BSU_32710

Isoelectric point: 4.62

Molecular weight: 28.88 kDa

Protein length: 261 aa

Gene length: 786 bp

Function: synthesis of Fe-S clusters

Product: ABC transporter (ATP-binding protein)

Essential: yes

Synonyms: yurY

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Regulatory ABC transporters
• Metabolism → Additional metabolic pathways → Iron metabolism → Biosynthesis of iron-sulfur clusters
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 3,359,995 → 3,360,780

Phenotypes of a mutant

• essential PubMed
• the mutant is viable and Fe-S clusters are dispensable if IspG and IspH from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate PubMed

The protein

Catalyzed reaction/ biological activity

• synthesis of Fe-S-clusters (the SufB-SufC-SufD complex) PubMed

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 6-248) (according to UniProt)

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Structure

• 5AWF (from E. coli, the SufB-SufC-SufD complex, 51% identity) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: sufC-sufD-sufS-sufU-sufB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• MGNA-B587 (yurY::erm), available at the NBRP B. subtilis, Japan

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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