atpH [2017-09-28 15:15:43]

ATP synthase, part of the F1 complex (subunit delta)

Locus: BSU36840

Isoelectric point: 9.57

Molecular weight: 19.84 kDa

Protein length: 181 aa

Gene length: 543 bp

Function: ATP synthesis

Product: ATP synthase (subunit delta)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → ATP synthesis → ATPase
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 3,785,403 → 3,785,948

The protein

Catalyzed reaction/ biological activity

• ATP synthesis see a video

Protein family

• ATPase delta chain family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-159 PubMed

Structure

• 5T4O (ATPase from E. coli, 25% identity) PubMed

Localization

• membrane (Heterogeneous) PubMed
• peripheral via theF0 complex

Expression and Regulation

Operons

• Genes: atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the mRNA is processed between atpI and atpB by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Mutant

• BKE36840 (ΔatpH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCGTTTAGAGACAGCTGATC, downstream forward: _UP4_AATCGATAGAAGGGGTGAAA
• BKK36840 (ΔatpH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCGTTTAGAGACAGCTGATC, downstream forward: _UP4_AATCGATAGAAGGGGTGAAA

References

Reviews

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Original publications

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