ykgA

similar to arginine-guanidine removing enzyme, general stress protein, survival of salt and ethanol stresses

locus: BSU_13020

pI: 4.83

mw: 29.49 kDa

protein length: 286 aa

gene length: 861 bp

function: survival of salt and ethanol stresses

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 1,371,015 → 1,371,875

The protein

Protein family

• DDAH family (single member, according to UniProt)

Structure

• [PDB|6LRG] (arginine-guanidine removing enzyme from Escherichia coli, corresponds to aa 23 ... 280, 26% identity) [pubmed|32198136]

Expression and Regulation

Operons

• genes: [gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]-[gene|491B5E27EF3190D307EC43C3BB12490728E39333|ykgB]
  description: [Pubmed|10482513]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10482513], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|10482513]

Biological materials

Mutant

• MGNA-A753 (ykgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/753 NBRP B. subtilis, Japan]
• BKE13020 (Δ[gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCTCCTAAA, downstream forward: _UP4_TGAAGAATCTTCCCCTTTTT
• BKK13020 (Δ[gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCTCCTAAA, downstream forward: _UP4_TGAAGAATCTTCCCCTTTTT

References

• 15805528,10482513,32198136