licA

lichenan-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIA of the [category|SW 1.2.2|PTS]

locus: BSU_38570

pI: 4.64

mw: 12.04 kDa

protein length: 110 aa

gene length: 333 bp

function: lichenan uptake and phosphorylation

product: lichenan-specific [category|SW 1.2.2|PTS], EIIA component

essential: no

synonyms: celC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 3,959,841 → 3,960,173

The protein

Protein family

• [category|SW 1.2.2|PTS] permease, lactose family [Pubmed|10627040]

Paralogous protein(s)

• [protein|44014896157718D3A6190DFDBD663C5CB9DA63FC|GmuA]

[SW|Domains]

• [SW|PTS EIIA domain] type-3 (aa 3-101) (according to UniProt)

Structure

• [PDB|3K1S] (IIA(Cel) from ''B. anthracis'', 42% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]-[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]-[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]-[gene|84D8AA0AFBF46273F6B4198B49440D55470FEC0F|licH]
  description: [Pubmed|8990303]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8990303], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8990303], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR]: activation, [Pubmed|8990303], in [regulon|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR regulon]

  regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8990303]

Biological materials

Mutant

• BKE38570 (Δ[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCACGCGATTCACTTCCT, downstream forward: _UP4_ACCGAGCAAAGGGGAGCAAG
• BKK38570 (Δ[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCACGCGATTCACTTCCT, downstream forward: _UP4_ACCGAGCAAAGGGGAGCAAG

References

• 19087206,8990303,16872404,10438772,10627040,8990303,27766092