moaB [2019-07-09 09:30:29]

molybdopterin precursor biosynthesis

locus: BSU29460

pI: 5.07

mw: 18.31 kDa

protein length: 170 aa

gene length: 513 bp

function: nitrate respiration

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.10|Biosynthesis of molybdopterin]

Gene

Coordinates: 3,014,514 → 3,015,026

The protein

Protein family

• moaB/mog family (according to Swiss-Prot)

Structure

• [PDB|1Y5E] (from B. cereus, 61% identity)

Expression and Regulation

Operons

• genes: [gene|E89A93C59339AB8814B92A2DF8C3FF95C5C940D3|moaB]
  description: [Pubmed|9387221]

Biological materials

Mutant

• MGNA-A115 (moaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/115 NBRP B. subtilis, Japan]
• BKE29460 (Δ[gene|E89A93C59339AB8814B92A2DF8C3FF95C5C940D3|moaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGGCCCACCTCTTTTAT, downstream forward: _UP4_TAAATGAATAAATACTATGA
• BKK29460 (Δ[gene|E89A93C59339AB8814B92A2DF8C3FF95C5C940D3|moaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGGCCCACCTCTTTTAT, downstream forward: _UP4_TAAATGAATAAATACTATGA

References

Reviews

• 23539623

Original publications

• 9387221