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walK [2018-12-02 13:08:08]
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walK [2018-12-02 13:08:08]

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors
locus
BSU40400
pI
4.78
mw
69.86 kDa
protein length
611 aa Sequence Blast
gene length
1833 bp Sequence Blast
function
control of cell wall metabolism
product
two-component sensor kinase (OmpR family)
essential
yes
ec
2.7.13.3
synonyms
yycG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    4,151,853 → 4,153,688

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • a mutant (point mutation) has been selected during growth at low pressure [Pubmed|26296725]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]

    • Protein family

  • two-component sensor kinase of the OmpR family

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|3SL2] (complex of the ATP binding histidine kinase domain with ATP) [Pubmed|22751669]

    • [SW|Localization]

  • cell membrane [Pubmed|21219466]
  • part of the division septum in growing cells, interacts with the [SW|divisome] [Pubmed|18573169]

    • Expression and Regulation

    Operons

    genes
    [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
    description
    [Pubmed|9829949]

    regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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    Biological materials

    Mutant

  • MGNA-B827 (yycG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1826 NBRP B. subtilis, Japan]

    • Expression vectors

  • pGP2636, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 19019149,18792692,10094672,18245295,28886686

    • Original Publications

  • 18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157,19342776,20057515,20167622,21219466,23632918,22751669,22526318,24490131,24900578,26296725,28674376