walK [2018-12-02 13:15:28]

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors

locus: BSU40400

pI: 4.78

mw: 69.86 kDa

protein length: 611 aa

gene length: 1833 bp

function: control of cell wall metabolism

product: two-component sensor kinase ([SW|OmpR family])

essential: yes

ec: 2.7.13.3

synonyms: yycG

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 4,151,853 → 4,153,688

Phenotypes of a mutant

• essential [Pubmed|12682299]
• essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
• a mutant (point mutation) has been selected during growth at low pressure [Pubmed|26296725]

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]

Protein family

• two-component sensor kinase of the [SW|OmpR family]

Paralogous protein(s)

• [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE], [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR]

[SW|Domains]

• two transmembrane segments
• [SW|PAS domain]
• C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

Structure

• [PDB|3SL2] (complex of the ATP binding histidine kinase domain with ATP) [Pubmed|22751669]

[SW|Localization]

• cell membrane [Pubmed|21219466]
• part of the division septum in growing cells, interacts with the [SW|divisome] [Pubmed|18573169]

Expression and Regulation

Operons

• genes: [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
  description: [Pubmed|9829949]

  regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]

Biological materials

Mutant

• MGNA-B827 (yycG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1826 NBRP B. subtilis, Japan]

Expression vectors

• pGP2636, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 19019149,18792692,10094672,18245295,28886686

Original Publications

• 18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157,19342776,20057515,20167622,21219466,23632918,22751669,22526318,24490131,24900578,26296725,28674376,29465029