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walK [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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walK [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors
locus
BSU40400
pI
4.00
mw
69.00 kDa
function
control of cell wall metabolism
product
two-component sensor kinase (OmpR family)
essential
yes
ec
2.7.13.3
synonyms
yycG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|cell wall/ other], [SW|protein modification], [SW|transcription factors and their control], [SW|essential genes], [SW|membrane proteins], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,151,853 -> 4,153,688

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • a mutant (point mutation) has been selected during growth at low pressure [Pubmed|26296725]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|search|WalR]

    • Protein family

  • two-component sensor kinase of the OmpR family

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|3SL2] (complex of the ATP binding histidine kinase domain with ATP) [Pubmed|22751669]

    • [SW|Localization]

  • cell membrane [Pubmed|21219466]
  • part of the division septum in growing cells, interacts with the [SW|divisome] [Pubmed|18573169]

    • [SW|Interactions]

  • [protein|search|WalK]-[protein|search|WalR]
  • [protein|search|WalK]-[protein|search|YycH] [Pubmed|21219466],
  • [protein|search|WalK]-[protein|search|YycI] [Pubmed|21219466]
  • [protein|search|WalK]-[SW|DivIB] [Pubmed|21219466]
  • [protein|search|WalK]-[protein|search|FtsL] [Pubmed|21219466]
  • [protein|search|WalK]-[protein|search|PbpB] [Pubmed|21219466]
  • [protein|search|WalK]-[protein|search|FtsZ] [Pubmed|18573169]

    • Expression and Regulation

    Operon

  • ''[protein|search|walR]-[protein|search|walK]-[protein|search|yycH]-[protein|search|yycI]-[protein|search|walJ]-[protein|search|htrC]'' [Pubmed|9829949]

    • Regulation

  • expressed during vegetative growth, repressed during stationary phase [Pubmed|9829949]

    • Biological materials

    Expression vector

  • pGP2636, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 19019149,18792692,10094672,18245295

    • Original Publications

  • 18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157,19342776,20057515,20167622,21219466,23632918,22751669,22526318,24490131,24900578,26296725