walK

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors

Locus: BSU_40400

Isoelectric point: 4.78

Molecular weight: 69.86 kDa

Protein length: 611 aa

Gene length: 1836 bp

Function: control of cell wall metabolism

Product: two-component sensor kinase

Essential: yes

E.C.: 2.7.13.3

Synonyms: yycG

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
• Groups of genes → Essential genes
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 4,151,853 → 4,153,688

Phenotypes of a mutant

• essential PubMed
• essentiality can be bypassed by constitutive expression of lytE or cwlO in a pdaC iseA mutant background PubMed
• a mutant (point mutation) has been selected during growth at low pressure PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of WalR
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Protein family

• two-component sensor kinase

Paralogous protein(s)

• ResE, PhoR

Domains

• two transmembrane segments
• HAMP domain (aa 204-256) (according to UniProt)
• PAS domain (aa 263-324) (according to UniProt)
• PAC domain (aa 325-379) (according to UniProt)
• Histidine kinase domain (aa 383-602) (according to UniProt)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• activity is modulated in response to changes in D,L-endopeptidase activity (via the extracellular PAS domain) PubMed

Structure

• 3SL2 (complex of the ATP binding histidine kinase domain with ATP) PubMed

Localization

• cell membrane PubMed
• part of the division septum in growing cells, interacts with the divisome PubMed

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC
  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B827 (yycG::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• pGP2636, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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