ylaN [Tue May 31 2016 11:41:31 GMT+0200 (CEST)]

unknown

locus: BSU14840

pI: 4.00

mw: 10.00 kDa

function: unknown

product: unknown

essential: yes

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

[SW|Categories] containing this gene/protein

• [SW|essential genes], [SW|proteins of unknown function]

Gene

Coordinates on the chromosome (coding sequence): 1,552,412 -> 1,552,693

Phenotypes of a mutant

• essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|27238023]

The protein

Protein family

• UPF0358 family (according to Swiss-Prot)

Structure

• [PDB|2ODM] [Pubmed|17469204]

Expression and Regulation

Operon

• ''[protein|search|ylaN]'' [Pubmed|22383849]

Biological materials

Expression vector

• IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
• IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Jörg Stülke]'s lab

References

• 17005971,17469204,22383849,23420519,27238023