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yabT [2019-01-30 09:43:08]
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yabT [2019-01-30 09:43:08]

Ser/Thr kinase, controls Translation and DNA integrity during spore development
Locus
BSU00660
Isoelectric point
9.94
Molecular weight
37.51 kDa
Protein length
338 aa Sequence Blast
Gene length
1017 bp Sequence Blast
Function
control of DNA integrity during spore development
Product
Ser/Thr kinase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
73,809 → 74,825

Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • phosphorylation of RecA on Ser-2 PubMed
  • phosphorylation of EF-Tu on Thr-63 during sporulation PubMed
  • phosphorylation of YabA on Thr-71 PubMed
  • Protein family

  • protein kinase domain (according to Swiss-Prot)
  • Effectors of protein activity

  • autophosphorylation is stimulated by non-specific binding to DNA PubMed
  • Structure

    Localization

  • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed
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    Biological materials

    Mutant

  • MGNA-B921 (yabT::erm), available at the NBRP B. subtilis, Japan
  • GP577 (erm), available in Jörg Stülke's lab
  • BKE00660 (ΔyabT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
  • BKK00660 (ΔyabT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
  • Expression vectors

  • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab, PubMed
  • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
  • LacZ fusion

  • translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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