SubtiBank SubtiBank
yabT [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yabT [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

Ser/Thr kinase, controls translation and DNA integrity during spore development
locus
BSU00660
pI
9.00
mw
37.00 kDa
function
control of DNA integrity during spore development
product
Ser/Thr kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|sporulation proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigF regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    73,809 -> 74,825

    Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development [Pubmed|23634894]

    • The protein

    Catalyzed reaction/ biological activity

  • phosphorylation of [protein|search|RecA] on Ser-2 [Pubmed|23634894]
  • phosphorylation of [tufA|search|EF-tu] on Thr-63 during [SW|sporulation] [Pubmed|26056311]

    • Protein family

  • [SW|protein kinase] domain (according to Swiss-Prot)

    • Effectors of protein activity

  • autophosphorylation is stimulated by non-specific binding to DNA [Pubmed|23634894]

    • [SW|Localization]

  • colocalizes strongly with the septal membrane separating the mother cells from the forespore [Pubmed|23634894]

    • [SW|Interactions]

  • [protein|search|YabT]-[protein|search|RecA] [Pubmed|23634894]
  • [protein|search|AbrB]-[protein|search|YabT] [Pubmed|24731262]
  • [tufA|search|EF-tu]-[protein|search|YabT] [Pubmed|26056311]

    • Expression and Regulation

    [SW|Sigma factor]

  • [protein|search|SigF] [Pubmed|16497325]

    • Regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]

    • Biological materials

    Mutant

  • GP577 (erm), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP391, available in [SW|Jörg Stülke]'s lab
  • purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP823, available in [SW|Jörg Stülke]'s lab
  • purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1408, available in [SW|Jörg Stülke]'s lab
    • translational lacZ fusion (in [protein|search|pAC7])
    pGP831, available in [SW|Jörg Stülke]'s lab

    References

    Reviews

  • 27148245

    • Original publications

  • 16497325,23634894,24731262,25278935,26056311