citR [2017-08-04 15:01:28]

transcriptional repressor of citA

locus: BSU09430

pI: 8.67

mw: 35.44 kDa

protein length: 308 aa

gene length: 924 bp

function: regulation of the minor citrate synthase

product: transcriptional repressor (LysR family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,020,073 → 1,020,948

The protein

Protein family

• [SW|LysR family]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]
  description: [Pubmed|8045898]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8045899], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• GP1283 (''[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
• GP1753 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''aphA3''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
• GP2360 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''erm''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

References

• 8045898,8045899