citR [2019-09-03 09:21:49]

transcriptional repressor of [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]

locus: BSU_09430

pI: 8.67

mw: 35.44 kDa

protein length: 291 aa

gene length: 876 bp

function: regulation of the minor citrate synthase

product: transcriptional repressor ([SW|LysR family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,020,073 → 1,020,948

The protein

Protein family

• [SW|LysR family] (according to UniProt)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]
  description: [Pubmed|8045898]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8045899], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• GP1283 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]'')::''aphA3'', available in [SW|Jörg Stülke]'s lab
• GP1753 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]-[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]'')::''aphA3'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
• GP2360 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]-[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA])''::''erm'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
• BKE09430 (Δ[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
• BKK09430 (Δ[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

Expression vectors

• pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab
• pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab
• pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
• pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
• pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
• pGP2262 (integration into [gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab

References

• 8045898,8045899