pyrAA

carbamoyl-phosphate synthetase (glutaminase subunit)

Locus: BSU_15510

Isoelectric point: 5.82

Molecular weight: 39.96 kDa

Protein length: 364 aa

Gene length: 1095 bp

Function: pyrimidine biosynthesis

Product: carbamoyl-phosphate synthetase (glutaminase subunit)

Essential: no

E.C.: 6.3.5.5

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides

Gene

Coordinates: 1,622,657 → 1,623,751

The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• CarA family (with CarA, according to UniProt)

Paralogous protein(s)

• CarA

Domains

• Glutamine amidotransferase type-1 domain (aa 171-356) (according to UniProt)

Structure

• 1JDB (from E. coli, 47% identity) PubMed

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

Biological materials

Mutant

• BKE15510 (ΔpyrAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC, downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
• BKK15510 (ΔpyrAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC, downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC

References

Reviews

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Original publications

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