ppsD

plipastatin synthetase

locus: BSU_18310

pI: 5.35

mw: 406.42 kDa

protein length: 3603 aa

gene length: 10812 bp

function: production of the antibacterial compound plipastatin

product: plipastatin synthetase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,964,045 → 1,974,856

The protein

Protein family

• [SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Paralogous protein(s)

• partial match (more than 1,000 amino acids): [protein|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|DhbF], [protein|13EB6B903088C11D985DDB7CEC864797058FE038|PksJ], [protein|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|PksN], [protein|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|PpsA], [protein|E7A2F93B33B1899313893D362D92405232BC9097|PpsB], [protein|C1C051209943E02369356075EAA733297E9478CB|PpsE], [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC]

[SW|Domains]

• 3 [SW|Carrier domain]s (aa 966-1041, aa 1997-2072, aa 3034-3108) (according to UniProt)

Structure

• [PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC], 38% identity) [pubmed|18583577]

Expression and Regulation

Operons

• genes: [gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]-[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]-[gene|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|ppsC]-[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]-[gene|C1C051209943E02369356075EAA733297E9478CB|ppsE]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• BKE18310 (Δ[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATGTCTCCTGTCA, downstream forward: _UP4_ATGCTCAGTTTTCACTAATG
• BKK18310 (Δ[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATGTCTCCTGTCA, downstream forward: _UP4_ATGCTCAGTTTTCACTAATG

References

• 10471562,9387222,22347779,20817675,18583577,29855381