lutR

transcriptional repressor ([SW|GntR family]) of the [gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|search|lutC ]operon and of [gene|E607B0D57398BCCF64CF3CE2110E632B0C3A109E|lutP]

locus: BSU_34180

pI: 4.84

mw: 19.50 kDa

protein length: 219 aa

gene length: 660 bp

function: control of lactate utilization

product: transcriptional repressor, ([SW|GntR family])

essential: no

synonyms: yvfI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,509,831 → 3,510,490

The protein

Catalyzed reaction/ biological activity

• transcriptional repressor of the [gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC] operon [Pubmed|19201793]

Protein family

• [SW|GntR family] of transcription factors

[SW|Domains]

• [SW|HTH gntR-type domain] (aa 1-56) (according to UniProt)

Effectors of protein activity

• lactate acts as molecular inducer [Pubmed|19201793]

Structure

• [PDB|6AZ6] ([protein|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|GntR] from Streptococcus agalactiae, 30% identity) [pubmed|29269393]

Expression and Regulation

Operons

• genes: [gene|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]
  description: [pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|25031425], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B607 (yvfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1606 NBRP B. subtilis, Japan]
• TEK1 lutR::Tn10::spc [Pubmed|24196425]
• BKE34180 (Δ[gene|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTAATAGGGCATCCG, downstream forward: _UP4_TAACCAACTCATTTCCCGGG
• BKK34180 (Δ[gene|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTAATAGGGCATCCG, downstream forward: _UP4_TAACCAACTCATTTCCCGGG

Expression vectors

• pQE60-lutR (His-tag) [Pubmed|24196425]

lacZ fusion

• TEK7 lutR::lacZ::erm [Pubmed|24196425]

labs

• [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
• [SW|Ayten Katatas], Istanbul Technical University, Istanbul, Turkey [http://www.bio.itu.edu.tr/personel_data/personel_ayten_yazgan_karatas.htm x]

References

• 24196425,19201793,25031425,18604637,29269393