SubtiBank SubtiBank
ilvB [Mon May 16 2016 16:56:39 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ilvB [Mon May 16 2016 16:56:39 GMT+0200 (CEST)]

acetolactate synthase (large subunit)
locus
BSU28310
pI
5.00
mw
62.00 kDa
function
biosynthesis of branched-chain amino acids
product
acetolactate synthase (large subunit)
essential
no
ec
2.2.1.6
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon], [SW|CcpA regulon], [SW|TnrA regulon], [SW|T-box]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,895,248 -> 2,896,972

    The protein

    Catalyzed reaction/ biological activity

  • 2 pyruvate = 2-acetolactate CO2 (according to Swiss-Prot)

    • Protein family

  • TPP enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|AlsS], [protein|search|YdaP]

    • Modification

  • phosphorylated on Arg-297 [Pubmed|22517742]

    • Structure

  • [PDB|4RJJ] [Pubmed|25393087]
  • [PDB|1N53] (T box RNA)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

    • Expression and Regulation

    Operon

  • ''[protein|search|ilvB]-[protein|search|ilvH]-[protein|search|ilvC]-[protein|search|leuA]-[protein|search|leuB]-[protein|search|leuC]-[protein|search|leuD]'' [Pubmed|1577690]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1577690]

    • Regulation

  • for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
  • expression is stimulated in the presence of glucose ([protein|search|CcpA]) [http://www.ncbi.nlm.ntnrih.gov/sites/entrez/12193635 PubMed]
  • repressed under nitrogen limiting conditions (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|15547269]
  • repressed by casamino acids [Pubmed|12107147]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription activation [Pubmed|12193635]
  • repression by BCAA: tRNA-controlled [SW|RNA switch] ([protein|search|T-box]) that mediates termination/antitermination
  • [protein|search|TnrA]: transcription repression [Pubmed|15547269]
  • [protein|search|CodY]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

    • Biological materials

    Mutant

  • GP324, ''ilvB'' under control of pXyl (cat), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP520 and pGP524 (both in [protein|search|pAC5]), pGP523 (in [protein|search|pAC6]), all are available in [SW|Jörg Stülke]'s lab

    • References

  • 21303765,15060025,19258532,8289305,17488331,18083814,18641142,24163341,15547269,12618455,12107147,1577690,12193635,15916605,15916606,20935095,22517742,22900538,25157083,25755103,26220295
  • [PDB|][PDB|]