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recQ [2020-09-29 09:57:02]
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recQ [2020-09-29 09:57:02]

ATP-dependent DNA helicase, acts together with RecJ in replication fork maintenance
Locus
BSU_19220
Isoelectric point
5.92
Molecular weight
67.18 kDa
Protein length
591 aa Sequence Blast
Gene length
1776 bp Sequence Blast
Function
replication fork maintenance
Product
ATP-dependent DNA helicase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,094,010 → 2,095,785

The protein

Catalyzed reaction/ biological activity

  • unwinds covalently closed ds DNA (in complex with SSB) PubMed
  • RecQ controls Topo III (de)catenation activity PubMed
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
  • Protein family

  • helicase family (according to UniProt)
  • Paralogous protein(s)

    Domains

  • Helicase ATP-binding domain (aa 27-196) (according to UniProt)
  • Helicase C-terminal domain (aa 216-367) (according to UniProt)
  • HRDC domain (aa 513-591) (according to UniProt)
  • Structure

  • 4Q47 (RecQ from Deinococcus radiodurans, 40% identity) PubMed
  • Localization

  • associated with the replisome PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • GP3543 (del kan), available in Jörg Stülke's lab
  • MGNA-B414 (yocI::erm), available at the NBRP B. subtilis, Japan
  • BKE19220 (ΔrecQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
  • BKK19220 (ΔrecQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
  • References

    Reviews

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    Original publications

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