malP

maltose-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIICB of the [category|SW 1.2.2|PTS]

locus: BSU_08200

pI: 8.87

mw: 57.89 kDa

protein length: 527 aa

gene length: 1584 bp

function: maltose uptake and phosphorylation

product: maltose-specific [category|SW 1.2.2|PTS], EIICB

essential: no

ec: 2.7.1.69

synonyms: glvC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 892,215 → 893,798

Phenotypes of a mutant

• no growth on maltose as single carbon source [Pubmed|10627040]

The protein

Catalyzed reaction/ biological activity

• D-maltose + N^π-phospho-L-histidyl-[protein] --> α-maltose 6'-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• [category|SW 1.2.2|PTS] permease, glucose family [Pubmed|10627040]

[SW|Domains]

• [SW|PTS EIIB domain] type-1 (aa 449–527) (according to UniProt)
• [SW|PTS EIIC domain] type-1 (aa 1-418) (according to UniProt)

Structure

• [PDB|6BVG] (EIIC domain from B. cereus, corresponds to aa 6 - 419, 27% identity) [pubmed|29784777]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
  description: [Pubmed|11489864]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489864], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR]: activation, [Pubmed|11489864], in [regulon|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|11489864], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]

Biological materials

Mutant

• MGNA-C291 (glvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2289 NBRP B. subtilis, Japan]
• GP110 (spc), available in [SW|Stülke] lab
• BKE08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
• BKK08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC

References

• 16707683,10627040,11489864,30038046,29784777