tatCY

component of the [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY] twin-arginine translocase

locus: BSU_05990

pI: 9.69

mw: 28.91 kDa

protein length: 254 aa

gene length: 765 bp

function: TAT [SW|protein secretion]

product: component of the twin-arginine translocation pathway

essential: no

synonyms: ydiJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 647,940 → 648,704

The protein

Catalyzed reaction/ biological activity

• secretion of [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|EfeB] and [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|YkuE]
• delivers [protein|D4AF96C936093BA44E46FF92A3EDE437495B0923|QcrA] to the membrane [Pubmed|23256564]

Protein family

• tatC family (with [protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|TatCD], according to UniProt)

Paralogous protein(s)

• [protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|TatCD]

Structure

• [PDB|4HTT] (from Aquifex aeolicus, 35% identity) [pubmed|23583035]

[SW|Localization]

• cell membrane (according to Swiss-Prot), Polar (Septum) [Pubmed|16479537]

Additional information

• [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY]-dependent export of [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|YkuE] and [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|EfeB] requires a functional [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|WprA] [Pubmed|23180473]

Expression and Regulation

Operons

• genes: [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
  description: [Pubmed|22383849]

  regulation

  • constitutitvely expressed [Pubmed|22383849]

• genes: [gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]-[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]-[gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
  description: [Pubmed|22383849]

  regulation

  • constitutitvely expressed [Pubmed|22383849]
  • the mRNA is processed between [gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex] and [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• BKE05990 (Δ[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAACTTACTGATCTT, downstream forward: _UP4_TAATAAAAAATCCATTTCTC
• BKK05990 (Δ[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAACTTACTGATCTT, downstream forward: _UP4_TAATAAAAAATCCATTTCTC

labs

• [SW|Jan Maarten van Dijl], University of Groningen, The Netherlands, [https://www.rug.nl/staff/j.m.van.dijl/ Homepage]

References

Reviews

• 22683878,24140208,25975269,25494301,27121927,31401776

Research papers

• 23583035

Original publications

• 11007775,15554971,22041895,16479537,19383693,19395490,21479178,22544248,22767609,22383849,23256564,24236045,24875903,23180473,26239117,32302670