nap [2019-08-27 09:26:29]

carboxylesterase NA

locus: BSU_05440

pI: 5.80

mw: 33.80 kDa

protein length: 300 aa

gene length: 903 bp

function: lipid degradation

product: carboxylesterase NA

essential: no

ec: 3.1.1.1

synonyms: cesA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.4|Utilization of lipids/ other]

Gene

Coordinates: 592,303 → 593,205

The protein

Catalyzed reaction/ biological activity

• carboxylic ester + H₂O --> alcohol + carboxylate (according to UniProt)

Protein family

• [SW|AB hydrolase superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|27FB777AEBCC794A5A768C7FE7D8374BCEA75913|YbfK]

Structure

• [PDB|2R11]

Expression and Regulation

Operons

• genes: [gene|E5A59EE56092F8042128B19D5E8DEFAE41C78000|nap]
  description: [Pubmed|12823818]

Biological materials

Mutant

• BKE05440 (Δ[gene|E5A59EE56092F8042128B19D5E8DEFAE41C78000|nap]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAATGCCCCTCCCAA, downstream forward: _UP4_TAAGGGCTTATAAGATAGTG
• BKK05440 (Δ[gene|E5A59EE56092F8042128B19D5E8DEFAE41C78000|nap]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAATGCCCCTCCCAA, downstream forward: _UP4_TAAGGGCTTATAAGATAGTG

References

• 12823818,24418394,22248594