yqhB

similar to magnesium exporter, general stress protein, required for protection against oxidative stress

Locus: BSU_24750

Isoelectric point: 5.54

Molecular weight: 49.79 kDa

Protein length: 442 aa

Gene length: 1329 bp

Function: protection against stress conditions

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Magnesium uptake/ efflux
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,561,585 → 2,562,913

The protein

Protein family

• UPF0053 family(according to UniProt)

Paralogous protein(s)

• YrkA, YugS, YhdT, MpfA

Domains

• CNNM transmembrane domain (aa 7-210) (according to UniProt)
• DUF21 domain (14-210)
• 2 CBS domains (aa 229-290, aa 295-355) (according to UniProt)

Structure

• 4HG0 (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yqhB
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-C474 (yqhB::erm), available at the NBRP B. subtilis, Japan
• BKE24750 (ΔyqhB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
• BKK24750 (ΔyqhB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC

Expression vectors

• pGP2935 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

References

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