SubtiBank SubtiBank
yqhB
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yqhB

similar to magnesium exporter, general stress protein, required for protection against oxidative stress
locus
BSU_24750
pI
5.54
mw
49.79 kDa
protein length
442 aa Sequence Blast
gene length
1329 bp Sequence Blast
function
protection against stress conditions
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,561,585 → 2,562,913

    The protein

    Protein family

  • [SW|UPF0053 family](according to UniProt)

    • Paralogous protein(s)

  • [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|YrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|MpfA]

    • [SW|Domains]

  • [SW|CNNM transmembrane domain] (aa 7-210) (according to UniProt)
  • [SW|DUF21 domain] (14-210)
  • 2 [SW|CBS domain]s (aa 229-290, aa 295-355) (according to UniProt)

    • Structure

  • [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]
    description
    [Pubmed|16267290]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C474 (yqhB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2472 NBRP B. subtilis, Japan]
  • BKE24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
  • BKK24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC

    • Expression vectors

  • pGP2935 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 16267290,15805528,22582280,27933050,31415562