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abrB [Tue Dec 29 2015 16:30:12 GMT+0100 (CET)]
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abrB [Tue Dec 29 2015 16:30:12 GMT+0100 (CET)]

transcriptional regulator of transition state genes
locus
BSU00370
pI
6.00
mw
10.00 kDa
function
regulation of gene expression during the transition
from growth to stationary phase
product
transcriptional regulator
essential
no
synonyms
cpsX, tolB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|transition state regulators]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    44,848 -> 45,138

    Phenotypes of a mutant

  • No swarming motility on B medium [Pubmed|19202088]

    • The protein

    Paralogous protein(s)

  • [protein|search|Abh], [SW|SpoVT] (only N-terminal domain)

    • Modification

  • phosphorylated on Ser-86 [Pubmed|20509597] by [protein|search|PrkC], [protein|search|PrkD], and [protein|search|YabT] results in loss of DNA-binding activity [Pubmed|24731262]

    • Effectors of protein activity

  • interaction with [protein|search|AbbA] results in inactivation of AbrB [Pubmed|18840696]

    • Structure

  • [PDB|1Z0R] (N-terminal DNA recognition domain), [Pubmed|16223496]
  • [PDB|2MJG] (full-length protein) [Pubmed|25308864]

    • [SW|Interactions]

  • [protein|search|AbbA]-[protein|search|AbrB], [protein|search|AbbA] binds the N-terminal DNA-binding domain of [protein|search|AbrB] [Pubmed|24534728,18840696]
  • [protein|search|AbrB]-[protein|search|AbrB] [Pubmed|20817675]
  • [protein|search|AbrB]-[protein|search|Abh] [Pubmed|20817675]
  • [protein|search|AbrB]-[protein|search|PrkC] [Pubmed|24731262]
  • [protein|search|AbrB]-[protein|search|PrkD] [Pubmed|24731262]
  • [protein|search|AbrB]-[protein|search|YabT] [Pubmed|24731262]
  • [SW|RnaC]-mRNA of ''[SW|abrB]'' to trigger degradation of the ''[SW|abrB]'' mRNA [Pubmed|25790031]

    • Expression and Regulation

    Operon

  • ''abrB'' [Pubmed|3145384]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|3145384]

    • Regulation

  • expressed at the onset of stationary phase [Pubmed|3145384]
  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/2106683,15687200 PubMed]
  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|2504584]

    • Regulatory mechanism

  • repressed by [SW|Spo0A]-P [Pubmed|3145384]
  • [SW|Spo0A]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/2106683,15687200 PubMed]
  • [protein|search|AbrB]: transcription repression [Pubmed|2504584]

    • Additional information

  • degradation of the ''[SW|abrB]'' mRNA is triggered by base-pairing with [SW|RnaC] [Pubmed|25790031]

    • Biological materials

    Mutant

  • TT731 (aphA3)
  • 1A935 (''abrB''::''cat''), [Pubmed|11886552], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A935&Search=1A935 BGSC]
  • GP1548 (''abrB''::''ermC''), available in [SW|Stülke] lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

    • Labs working on this gene/protein

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
  • [SW|Mark Strauch], Baltimore, USA [http://lifesciences.umaryland.edu/Pages/faculty_profile.aspx?ID=212 homepage]

    • References

    Reviews

  • 11964117,19995980,11931565,19202088,18326573

    • The [SW|AbrB regulon]:

  • 20817675,18840696,23580539

    • Other original publications

  • 19581368,2437099,8878039,11377867,1850083,12586407,2554317,18430133,7768874,2507867,1766371,15687200,1908787,2106683,15687200,2504584,3145384,8821944,8576231,11101897,11395475,11583849,11751836,12123659,12076816,12591885,15610005,16223496,16159768,16702211,17660417,17720793,7592460,19465659,20509597,15101989,19202088,18326573,24534728,24731262,24832089,25002359,25308864,25381239,25790031