yitB [2019-07-31 08:28:11]

phospho-adenylylsulfate sulfotransferase

Locus: BSU_10930

Isoelectric point: 5.77

Molecular weight: 27.52 kDa

Protein length: 236 aa

Gene length: 711 bp

Function: sulfate reduction and activation

Product: phospho-adenylylsulfate sulfotransferase

Essential: no

E.C.: 1.8.4.8

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Sulfur metabolism
• Metabolism → Additional metabolic pathways → Sulfur metabolism → sulfur metabolism/ general
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,171,755 → 1,172,465

The protein

Catalyzed reaction/ biological activity

• Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)

Protein family

• PAPS reductase family (with CysH, according to UniProt)

Paralogous protein(s)

• CysH

Structure

• 2OQ2 (from yeast, 33% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yitB-yitA-yisZ
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulation

  • expressed during sporulation in the mother cell (SigK) PubMed

Biological materials

Mutant

• MGNA-A504 (yitB::erm), available at the NBRP B. subtilis, Japan
• BKE10930 (ΔyitB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
• BKK10930 (ΔyitB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG

References

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