mcpB [2018-02-11T17:14:13.000Z]

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine

locus: BSU31260

pI: 5.13

mw: 71.70 kDa

protein length: 662 aa

gene length: 1986 bp

function: control of chemotaxis

product: methyl-accepting chemotaxis protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.4|Membrane-bound chemoreceptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,210,445 → 3,212,433

The protein

Paralogous protein(s)

• [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|McpA]

Kinetic information

• K(D) for asparagine: 14 myM [Pubmed|19864420]

Modification

• methylated at residues Gln371, Glu630, and Glu637 [Pubmed|20864474]

Effectors of protein activity

• asparagine (binds to the part of McpB that is exposed to the exterior) [Pubmed|19864420]

[SW|Localization]

• cell membrane [Pubmed|18763711,21515776]
• localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) [Pubmed|21098025]

Expression and Regulation

Operons

• genes: [gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]
  description: [Pubmed|8188684]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8188684], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, McpB is present with 6,200 /- 1,900 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• BKE31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG, downstream forward: _UP4_TAAAAACCGAAAAACAGCGC
• BKK31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG, downstream forward: _UP4_TAAAAACCGAAAAACAGCGC

References

• 11722727,10825179,8251536,12909020,10196193,15544802,6137212,9194713,2505839,8188684,2105313,12123464,14731274,20864474,18763711,19864420,21098025,21515776,25039821,27899502