snaA

sulphur N-acetylase, required for the conversion of S-methyl-cysteine to cysteine

Locus: BSU_29390

Isoelectric point: 5.51

Molecular weight: 20.32 kDa

Protein length: 178 aa

Gene length: 537 bp

Function: utilization of S-methyl-cysteine

Product: sulphur N-acetylase

Essential: no

Synonyms: ytmI

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Sulfur metabolism → Conversion of S-methyl cysteine to cysteine

Gene

Coordinates: 3,008,249 → 3,008,785

Phenotypes of a mutant

• reduced growth with S-methyl cysteine PubMed
• the snaA snaB double mutant does not grow with S-methyl cysteine PubMed

The protein

Catalyzed reaction/ biological activity

• N-acetylation of the amino group of S-methyl cysteine PubMed

Protein family

• Acetyltransferase family (according to UniProt)

Paralogous protein(s)

• SnaB

Domains

• N-acetyltransferase domain (aa 4-163) (according to UniProt)

Structure

• 4ZBG (from Brucella melitensis, 24% identity)

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

  Regulation

  • induced in the presence of methionine and taurine PubMed

Biological materials

Mutant

• MGNA-A156 (ytmI::erm), available at the NBRP B. subtilis, Japan
• BKE29390 (ΔsnaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTGTCACTCCTCTTC, downstream forward: _UP4_TGATGTGAGGCAGCCTATGA
• BKK29390 (ΔsnaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTGTCACTCCTCTTC, downstream forward: _UP4_TGATGTGAGGCAGCCTATGA

References

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