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ppsA [2018-02-11T01:21:16.000Z]
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ppsA [2018-02-11T01:21:16.000Z]

plipastatin synthetase
locus
BSU18340
pI
5.22
mw
288.86 kDa
protein length
2561 aa Sequence Blast
gene length
7683 bp Sequence Blast
function
production of the antibacterial compound plipastatin
product
plipastatin synthetase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    1,990,272 → 1,997,957

    The protein

    Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • shares highly conserved domains of more than 1,000 amino acids with: [protein|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|DhbF], [protein|13EB6B903088C11D985DDB7CEC864797058FE038|PksJ], [protein|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|PksN], [protein|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|PpsA], [protein|E7A2F93B33B1899313893D362D92405232BC9097|PpsB], [protein|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|PpsC], [protein|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|PpsD], [protein|C1C051209943E02369356075EAA733297E9478CB|PpsE], [protein|81845F44CE2C601555066E31E87384FF5D7B4139|SrfAA], [protein|3C484FDB3A440269E87D6B6E8500A875AC456666|SrfAB], [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC]

    • Structure

  • [PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC], 30% identity) [pubmed|18583577]

    • Expression and Regulation

    Operons

    genes
    [gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]-[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]-[gene|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|ppsC]-[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]-[gene|C1C051209943E02369356075EAA733297E9478CB|ppsE]
    description
    [Pubmed|20817675]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
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    Biological materials

    Mutant

  • BKE18340 (Δ[gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC, downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT
  • BKK18340 (Δ[gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC, downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT

    • References

  • 10471562,9387222,20817675,18583577