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ccpA

Carbon catabolite control protein A, involved in glucose regulation of many genes; represses catabolic genes and activates genes involved in excretion of excess carbon

locus

BSU_29740

5.06

36.78 kDa

protein length

334 aa Sequence Blast

gene length

1005 bp Sequence Blast

function

carbon catabolite repression (CCR)

product

transcriptional regulator ([SW|LacI family])

essential

synonyms

graR,alsA,amyR

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

Gene

Coordinates

3,044,165 → 3,045,169

Phenotypes of a mutant

The mutant is unable to grow on a minimal medium with glucose and ammonium as the only sources of carbon and nitrogen, respectively, this can be suppressed by mutations resulting in hyperactive [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topoisomerase I] or in the inactivation of [gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [pubmed|29242163,17183217]

The protein

Catalyzed reaction/ biological activity

transcriptional regulator of carbon catabolite repression (CCR)

Protein family

[SW|LacI family]

[SW|Domains]

[SW|HTH lacI-type domain] (aa 1-58) (according to UniProt)

DNA binding Domain (6 – 25)

[SW|Cofactors]

[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]-Ser46-P, [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|Crh]-Ser-46-P

Effectors of protein activity

glucose-6-phosphate, fructose-1,6-bisphosphate [Pubmed|17376479]

Structure

[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and a optimal synthetic operator site)

[PDB|2HSG] (apoprotein) [pubmed|17500051]

CcpA-[protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|Crh]-DNA-complex [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI]

complex with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and sulphate ions [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39857 NCBI]

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000022 PRODORIC2 database]

Expression and Regulation

Operons

genes

description

[Pubmed|16547058]

sigma factors

[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

regulation

constitutive

view in new tab

additional information

there are about 3.000 molecules of CcpA per cell [http://www.ncbi.nlm.nih.gov/sites/entrez/8000527 PubMed], this corresponds to a concentration of 3 µM (according to [PubMed|20408793])

Biological materials

Mutant

QB5407 (ccpA::spc) [Pubmed|10941796], available in [SW|Jörg Stülke]'s lab

GP302 (erm) [Pubmed|12123463], available in [SW|Jörg Stülke]'s lab

GP300 (an in frame deletion of ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'') [Pubmed|11557150], available in [SW|Jörg Stülke]'s lab

WH649 (aphA3), available in [SW|Gerald Seidel]'s lab

BKE29740 (Δ[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAAAACCACTCCTTT, downstream forward: _UP4_TAAGAAAAACAAAGAGCAAG

BKK29740 (Δ[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAAAACCACTCCTTT, downstream forward: _UP4_TAAGAAAAACAAAGAGCAAG

Expression vectors

pGP643 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pWH940 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Gerald Seidel]'s lab

Antibody

available in [SW|Gerald Seidel]'s and in [SW|Jörg Stülke]'s lab

labs

[SW|Gerald Seidel], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

[SW|Milton H. Saier], University of California at San Diego, USA [http://biology.ucsd.edu/faculty/saier.html Homepage]

[SW|Yasutaro Fujita], University of Fukuyama, Japan

[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

[SW|Oscar Kuipers], University of Groningen, The Netherlands [http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

20408793,8598282,19202299,14665673,18628769,18359269

General and physiological studies

1904524,10941796,12123463,8000527,18757537,16547058,14523131,22001508,22512862,29242163,30096425

Global analyses (proteome, transcriptome, ChIP-chip)

12850135,11251851,10559165,11160890,17183215,22383848,22900538,26483775

Repression of target genes by CcpA

15150224,16166551,11929549,7913927,17827291,11985717,12100558,7592486,16825793,16491025,21398533,26712933,28974613

Positive regulation of gene expression by CcpA

8226682,12193635,10559153,15916605,9811655,10986270,25157083

Control of CcpA activity

7623661,9973552,9334231,12051938,9689125

CcpA-DNA interaction

8596444,10666464,15885105,7665492,9254709,21106498

Functional analysis of CcpA

10383986,10601226,11557150,9252590,9988473

Structural analyses

15369672,16316990,17376479,16755587,17500051,17401189,10666630