primary bacitracin resistance determinant, ABC transporter (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system
function
export of toxic peptides
product
bacitracin ABC transporter (permease)
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,109,397 → 3,111,337
Phenotypes of a mutant
85-fold increased sensitivity to bacitracin [Pubmed|26815905]The protein
Catalyzed reaction/ biological activity
binds bacitracin, KD: 60 nM [Pubmed|25118291]inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|BceS] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]Protein family
ABC-4 integral membrane protein family (according to Swiss-Prot)Effectors of protein activity
the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199][SW|Localization]
cell membrane (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
description
[Pubmed|12890034]
regulatory mechanism
[protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]regulation
induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]view in new tabBiological materials
Mutant
MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]References
Reviews
Original publications
18394148,17905982,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,25118291,24806199,26199330,26815905