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mutS [Sun Dec 11 2016 09:58:39 GMT+0100 (CET)]
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mutS [Sun Dec 11 2016 09:58:39 GMT+0100 (CET)]

DNA mismatch repair (mismatch recognition protein)
locus
BSU17040
pI
5.00
mw
97.00 kDa
function
DNA repair
product
mismatch recognition protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,775,745 -> 1,778,321

    Phenotypes of a mutant

  • 60-fold increased mutation rate [Pubmed|23882084]
  • a ''[protein|search|rnhB] [protein|search|mutS]-[protein|search|mutL]'' mutant is not viable [Pubmed|23882084]

    • The protein

    Catalyzed reaction/ biological activity

  • MutS recognizes mismatches in the DNA and recruits [protein|search|MutL] to the site of mismatch recognition

    • Protein family

  • DNA mismatch repair mutS family (according to Swiss-Prot)

    • Structure

  • [PDB|1NG9] (from ''E. coli'', 39% identity) [Pubmed|12554674]

    • [SW|Localization]

  • forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]
  • replication-active region of the DNA, via [protein|search|DnaN] [Pubmed|23228104]
  • MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the [SW|replisome] region, even in the absence of appreciable mismatch formation [Pubmed|26575623]

    • [SW|Interactions]

  • forms dimers [Pubmed|23998896]
  • [protein|search|MutS]-[protein|search|DnaN] [Pubmed|20453097]
  • [protein|search|DnaE]-[protein|search|MutS] [Pubmed|21958350]
  • [protein|search|MutL]-[protein|search|MutS] [Pubmed|23998896]

    • Expression and Regulation

    Operon

  • ''[protein|search|mutS]-[protein|search|mutL]'' [Pubmed|8760914]

    • Additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mutS]' [PubMed|20525796]
  • intracellular concentration: about 80 dimers (100 nM) [PubMed|23998896]

    • Biological materials

    Mutant

  • GP1190 (del ''[protein|search|mutS]-[protein|search|mutL]''::''aphA3'') [Pubmed|22178973], available in [SW|Jörg Stülke]'s lab
  • 1A833 ( ''mutS''::''spec''), [Pubmed|11779496], available at [hhttp://bgsc.org/getdetail.php?bgscid=1A833 Bacillus Genetic Stock Center]
  • BKE17040 (''mutS''::''erm trpC2''), available at [hhttp://bgsc.org/ Bacillus Genetic Stock Center]

    • References

    Reviews

  • 22933559,26354434,24629484,26343983

    • Original publications

  • 8760914,15375129,16479537,20525796,20453097,23228104,21958350,23882084,23998896,24062730,12554674,25326311,26163658,26575623