hmp

flavohemoglobin, involved in resistance to nitric oxide (NO), essential for long-term survival under anaerobic conditions

locus: BSU_13040

pI: 5.70

mw: 44.57 kDa

protein length: 399 aa

gene length: 1200 bp

function: resistance to NO

product: flavohemoglobin

essential: no

synonyms: ykiA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 1,372,792 → 1,373,991

The protein

Catalyzed reaction/ biological activity

• NADPH + 2 nitric oxide + 2 O₂ --> H⁺ + NADP⁺ + 2 nitrate (according to UniProt)
• NADH + 2 nitric oxide + 2 O₂ --> H⁺ + NAD⁺ + 2 nitrate (according to UniProt)

Protein family

• globin family (single member, according to UniProt)
• C-terminal part: flavoprotein pyridine nucleotide cytochrome reductase family (single member, according to UniProt)

[SW|Domains]

• [SW|FAD-binding FR-type domain] (aa 152-255) (according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|1CQX] (from Ralstonia eutropha, 47% identity) [pubmed|8557026]

Expression and Regulation

Operons

• genes: [gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]
  description: [Pubmed|8682784]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8682784], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10972836], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|16885456], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

• genes: [gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]-[gene|5688E6CB20A55856F4A4D893CED3D54BA21209AE|ykjA]
  description: [Pubmed|22383849]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

Biological materials

Mutant

• BKE13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC
• BKK13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC

References

• 8682784,15231799,24214949,10972836,16885456,18487332,16923910,22383849,28439033,8557026