hmp

flavohemoglobin, involved in resistance to nitric oxide (NO), essential for long-term survival under anaerobic conditions

Locus: BSU_13040

Isoelectric point: 5.70

Molecular weight: 44.57 kDa

Protein length: 399 aa

Gene length: 1200 bp

Function: resistance to NO

Product: flavohemoglobin

Essential: no

Synonyms: ykiA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Electron transport/ other
• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 1,372,792 → 1,373,991

The protein

Catalyzed reaction/ biological activity

• NADPH + 2 nitric oxide + 2 O₂ --> H⁺ + NADP⁺ + 2 nitrate (according to UniProt)
• NADH + 2 nitric oxide + 2 O₂ --> H⁺ + NAD⁺ + 2 nitrate (according to UniProt)

Protein family

• globin family (single member, according to UniProt)
• C-terminal part: flavoprotein pyridine nucleotide cytochrome reductase family (single member, according to UniProt)

Domains

• FAD-binding FR-type domain (aa 152-255) (according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 1CQX (from Ralstonia eutropha, 47% identity) PubMed

Expression and Regulation

Operons

• Genes: hmp
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • NsrR: repression, PubMed, in NsrR regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

• Genes: hmp-ykjA
  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE13040 (Δhmp::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC
• BKK13040 (Δhmp::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC

References

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