manA [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

mannose-6-phosphate isomerase, required for proper cell wall synthesis

locus: BSU12020

pI: 5.28

mw: 35.00 kDa

function: mannose utilization

product: mannose-6-phosphate isomerase

essential: no

synonyms: yjdE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.9|Utilization of mannose]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|ManR regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,274,692 -> 1,275,639

Phenotypes of a mutant

• cells lose the characteristic rod shape typical of wild type ''B. subtilis'' cells and instead appear as elongated spheres, which are significantly larger than normal [Pubmed|20862359]

Expression and Regulation

Operon

• ''[protein|search|manP]-[protein|search|manA]-[protein|search|yjdF]'' [Pubmed|20139185]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|20139185]

Regulation

• induced by mannose ([protein|search|ManR]) [Pubmed|20139185]
• repressed by glucose ([protein|search|ManR]) [Pubmed|20139185]
• strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]

Regulatory mechanism

• [protein|search|ManR]: transcription activation in the presence of mannose and absence of glucose [Pubmed|20139185]

References

• 10960106,20139185,10627040,20862359,23033921,26238998